Layers were fed basal diets (Control) or basal diets supplemented with 0.5% Ginkgo-leaves fermented with Candida utilis (CF group), Aspergillus niger (AF group), or their combined fermentation (CAF group), respectively, for an 8-week feeding trial. Compared with the control group, AF and CAF supplementa- tion improved (Po0.05) laying rate and feed conversion ratio. Birds fed FGL (CF, AF and CAF) supplemented diet had a decrease (Po0.05) in cracked-egg rate, egg-yolk choles-terol, serum total cholesterol, triglyceride and low density lipoprotein cholesterol levels, while serum high density lipoprotein cholesterol concentrations of birds from CF and AF groups were increased (Po0.05) compared with that of the control.