DNA concentration was determined by realtime PCR with the
Rotor-Gene Q (Qiagen, Hilden, Germany) in combination with the
Investigator Quantiplex Kit (Qiagen, Hilden, Germany) and the
Rotor-Gene software 2.0.2.4 according to manufacturer’s instructions.
PCR reactions were set up by mixing 2 ml of sample with 8 ml
Reaction Mix FQ and 8 ml Primer Mix IC FQ.