YMC Carotenoid is C30 bonded silica based reversed-phase column. The separation of geometric and positional isomers is a challenging task in reversed-phase chromatography. Subtle molecular differences have to be recognized and resolved by the particular stationary phase. Sander et al. have shown conclusively that polymeric C30 HPLC stationary phases are able to discriminate isomeric structures of long chain molecules [1, 2].