TPC was estimated by the Folin–Ciocalteu method described
by Kumaret al.[15]. To 200 mL of algal extracts at 1000mg/mL
were added 1.4 mL distilled water, 100mL Folin–Ciocalteu
reagent and 300 mL sodium carbonate (20%). After 30 min
incubation at room temperature in the dark, absorbance was
read at 760 nm in a microplate reader (Biochrom Asys UVM
340).
TPC was calculated based on the standard curve of gallic acid
at a concentration range of (0.005–0.500) mg/mL, and the re-sults are expressed in milligrams (mg) of gallic acid equivalent/g
extract.