Juvenile lemon sharks were subjected to a series of four treatments
with a minimum rest period of four days between trials. In
all cases the subject animal resumed feeding the same day, or the
day following a trial. Prior to a trial the water level in the tank was
lowered to a depth of approximately 60 cm to facilitate the capture
and handling of the animals. During previous laboratory experiments
it was observed that shark swimming speed was temporarily elevated
immediately following a change in water level; as such, a 24 h acclimation
period was established between the lowering of the water level
and the trial. All eight sharkswere subjected to four treatments in a random
order and each shark was subjected to an individual treatment
only once.