scenario varied from 14% to 13% (Table 2). The maximum amount
of bioenergy production was also achieved by the 4th scenario
(0.56 109 GJ/yr). In scenario 0, where anaerobic digestion of dairy
manure was only considered for bioenergy production,
0.32 109 GJ energy/yr could be produced. Therefore, use of algae
for dairy nutrient utilization and bioenergy production could
increase the bioenergy production by at least 64% (realistic case).
Perusal of Fig. 2, it was also clear that as compared to scenario 0,
in other scenarios bioenergy production was increased till 3rd
cycle and it was aroused because of internal nutrient recycling
which in turn increased bioenergy production.
It was observed that in the 3rd scenario, where enzymatic
hydrolysis of biomass was used to recover additional nutrients
and sugar, a low biomass concentration was achieved (2 g/L, 30%
lipid content) that had a negative impact on the overall bioenergy
production as compared to 2nd scenario. Therefore, this study
emphasizes that enhancing biomass processing may not always
increase the bioenergy production. It also depends on the efficacy
of the process itself. Therefore, to increase bioenergy production
in the 3rd scenario, it is required to increase the algal biomass
scenario varied from 14% to 13% (Table 2). The maximum amountof bioenergy production was also achieved by the 4th scenario(0.56 109 GJ/yr). In scenario 0, where anaerobic digestion of dairymanure was only considered for bioenergy production,0.32 109 GJ energy/yr could be produced. Therefore, use of algaefor dairy nutrient utilization and bioenergy production couldincrease the bioenergy production by at least 64% (realistic case).Perusal of Fig. 2, it was also clear that as compared to scenario 0,in other scenarios bioenergy production was increased till 3rdcycle and it was aroused because of internal nutrient recyclingwhich in turn increased bioenergy production.It was observed that in the 3rd scenario, where enzymatichydrolysis of biomass was used to recover additional nutrientsand sugar, a low biomass concentration was achieved (2 g/L, 30%lipid content) that had a negative impact on the overall bioenergyproduction as compared to 2nd scenario. Therefore, this studyemphasizes that enhancing biomass processing may not alwaysincrease the bioenergy production. It also depends on the efficacyof the process itself. Therefore, to increase bioenergy productionin the 3rd scenario, it is required to increase the algal biomass
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