As each sample is measured in triplicate, a median or average
can be calculated. The technical guidance document (Beaufort et al.,
2008) requires the calculation of the median rather than the
average number. Therefore, the median of each replicate sample
was calculated. The relative increase in time was determined by
calculating the logarithmic value of the result at day 0, log N0, and
subtracting it from the logarithmic value of each of the calculated
medians of the following testing days, log Nt. Showing the relative
increase in the charts in paragraph 3 enables comparison of growth
of L. monocytogenes between the tested products and to the
calculated maximum increase criterion of 3.4 log CFU/g. For products
that were tested more than once, for each product, the average
of the relative increase for each day was calculated.
As each sample is measured in triplicate, a median or averagecan be calculated. The technical guidance document (Beaufort et al.,2008) requires the calculation of the median rather than theaverage number. Therefore, the median of each replicate samplewas calculated. The relative increase in time was determined bycalculating the logarithmic value of the result at day 0, log N0, andsubtracting it from the logarithmic value of each of the calculatedmedians of the following testing days, log Nt. Showing the relativeincrease in the charts in paragraph 3 enables comparison of growthof L. monocytogenes between the tested products and to thecalculated maximum increase criterion of 3.4 log CFU/g. For productsthat were tested more than once, for each product, the averageof the relative increase for each day was calculated.
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