3.3. Water-soluble vitamins in honey
The sample analytes were identified by comparison to the retention times of WSV solutions and the attribution was confirmed by spiking the peak in the real sample with a standard solution containing known amounts of pure vitamin. To determine the retention times the reference standards were injected both individually and as a mixture. Quantification was performed by the external standard method based on peak areas of the eluted WSV.
For each vitamin, four concentration levels inside the relevant linearity interval were chosen.