The threat of imminent global warming coupled with diminishing crude oil reserves worldwide and rising energy demands entail the urgent need to replace fossil-based fuels with green biofuels. Biomass-based fuels, such as bioethanol, provide a promising alternative, since their energy is already included in the global carbon cycle, implying a significant reduction in carbon dioxide release (Karagöz และคณะ, 2012). Among biomass feedstock, lignocellulosic residues offer attractive renewable sources for bioethanol production as they do not compete with the food industry and represent the most abundant carbohydrate reserve worldwide (Saha และคณะ, 2013). In many countries, including Spain, crop straw is a plentiful by-product from agricultural activities, wheat straw having been selected as the raw material in the present study.