An improved tissue culture protocol has been developed in this report from the nodal segments selected from a 4 year old mature plant of M. citrifolia. This is the first report on multiplication of shoots in liquid culture system for Noni plant with good rate of
shoot multiplication. All the shoots were rooted ex vitro, which reduced the time, energy and cost of production of plantlets in vitro. About 100% plantlets were survived under natural conditions after hardening in the greenhouse. The procedure defined can be used for conservation strategy and mass multiplication of Noni genotypes.