EHL–PEG was prepared from enzymatic hydrolysis lignin to improve enzymatic hydrolysis of lignocelluloses. While with the increase of lignin content, EHL–PEG improved enzymatic hydrolysis of microcrystalline cellulose more obviously than PEG4600. Glucose yield of corn stover was increased from 16.7% to 70.1% by EHL–PEG, while PEG4600 used alone was 52.3%. EHL–PEG reduced 88% of the non-productive adsorption of cellulase on lignin film measured by QCM-D, while reduction with PEG4600 was 43%. EHL–PEG interacted with cellulase and dispersed cellulase aggregates to form smaller aggregates, thereby, reduced significantly nonproductive adsorption of cellulase on lignin and enhanced enzymatic hydrolysis of lignocelluloses.