The pigs were removed from feed and fasted for approximately 16 h before scanning to minimise interference from dietary residues. Immediately prior to scanning the pigs were weighed and then transferred to the DXA facility. They were injected intramuscularly with Stresnil (azaperone 40 mg/mL, Stresnil Neuroleptic Injection for Pigs, Ausrichter Pty Ltd.,NSW) at 2 mL/10 kg LW. When sufficiently sedated the pigs were transferred to the DXA scanner (Norland XR46 Densitometer machine).