3.2. Effects of developed PEF and OMF combination on extension ofsupercoolingFig. 4 show the results of the chicken breast sample temperatureprofile under the newly developed, combined PEF and OMF treatment protocol. The temperature profile of the chicken breastswithout treatments (non-treated sample) is also displayed. Notethere is no difference in the cooling rates between treated and nontreated chicken breast samples prior to ice nucleation of the nontreated sample. When the minimum current threshold as determined prior was detected during the cooling phase (0.024 A), PEF