Optimization process of bioethanol using Empty Fruit Bunch (EFB) was conducted in the area of hydrolysis and
fermentation. Enzymatic hydrolysis and fermentation were studied in the two variation methods, Simultaneous
Saccharification and Fermentation (SSF) and Separate Hydrolysis and Fermentation (SHF). Using 40 FPU of
concentration enzyme, it could be produce 4.74% of ethanol in 72 hour fermentation by SHF process and 6.05% of
ethanol in 24 hour by SSF process. From this study, the SSF method was considered as a better process than SHF
due to rapidly ethanol production and the highest concentration of produced ethanol.