After a range – finding test, the concentrations prepared for the experiment were 0 mg/L, 20 mg/L, 60 mg/L, 80 mg/L and 110 mg/L, with two replicates as described by Rahman et al., (2002).The amount of Paraquat dichloride which contained the require mg of Paraquat was determined from the 276 g/L of Paraquat dichloride formulation