Wild-derived materials were also employed:
an interspecific hybrid obtained
from the cross L. esculentum × L. peruvianum
PI-143679 (F1-per) by using pollen
mixture and embryo culture techniques,
and a second backcross to tomato derived
from the cross L. esculentum × L. chilense
LA 1938 (BC2-chil).
In field screening, advanced tomato
breeding lines derived from L. chilense LA
1932 and LA 1938 (5°-8° backcross) were
also used.
In interspecific crosses, the tomato line
FC, carrier of the recessive marker gene c
(potato leaf), was used as a female parent.
This line was also used as a TYLCV-susceptible
control in all assays.