3.4. Fatty acid composition of eggs, embryos and gonads during gametogenesis
ANOSIM analysis revealed significant differences between treatments when all the tissues (gonads, egg and embryos) were pooled (P = 0.01), and this is clearly shown in the nMDS plot in Fig. 2. Nonetheless, when tissues within each treatment were analyzed by one-way ANOSIM followed by pair-wise test, only overall FA signatures of gonads and embryos within the Pellet diet were found to be significantly different (P = 0.02). The SIMPER test showed that the only LC-PUFA involved in the observed difference was ARA, which was clearly accumulated in the eggs and further retained during embryo development in the Pellet treatment (Table 4). Moreover EPA was significantly higher in eggs and embryos produced by urchins fed with Kelp than in those derived from the broodstock fed with the Pellet diet, while the opposite was true for DHA, following a common pattern observed in the gonads. Interestingly 20:2 and 20:3 NMI FAs were also accumulated in the eggs from urchins fed with both dietary treatments although eggs and embryos derived from the Pellet treatment had significantly higher 20:2 and 20:3 NMI compared with those of the Kelp treatment (Table 4).
3.4. Fatty acid composition of eggs, embryos and gonads during gametogenesisANOSIM analysis revealed significant differences between treatments when all the tissues (gonads, egg and embryos) were pooled (P = 0.01), and this is clearly shown in the nMDS plot in Fig. 2. Nonetheless, when tissues within each treatment were analyzed by one-way ANOSIM followed by pair-wise test, only overall FA signatures of gonads and embryos within the Pellet diet were found to be significantly different (P = 0.02). The SIMPER test showed that the only LC-PUFA involved in the observed difference was ARA, which was clearly accumulated in the eggs and further retained during embryo development in the Pellet treatment (Table 4). Moreover EPA was significantly higher in eggs and embryos produced by urchins fed with Kelp than in those derived from the broodstock fed with the Pellet diet, while the opposite was true for DHA, following a common pattern observed in the gonads. Interestingly 20:2 and 20:3 NMI FAs were also accumulated in the eggs from urchins fed with both dietary treatments although eggs and embryos derived from the Pellet treatment had significantly higher 20:2 and 20:3 NMI compared with those of the Kelp treatment (Table 4).
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