QuantiTect Probe PCR master mix (Qiagen), 900 nM forward
primer (GAA CTC AGT GTA GGT AGA ATG TTT GCA),
300 nM reverse primer (TTC AGC TAT CAT TTT CTC TGC
CAA T) and 100 nM probe (6FAM-TTTGAACCTGTCTGAAC
ATTCCCGGTT-TAMRA) were used. The limit of detection was
10 copies of RSV-L gene. Quantification was performed from
standard curves of a pcDNA3 plasmid carrying a fragment of
the RSV L-gene. Results are expressed as RSV-L gene copy
numbers and transformed in log10 values. The antiviral effect of
TMC353121 is expressed as median log reduction versus
untreated control (Dlog10).