2.6. Determination of the total phenolics content
2.6.1. Preparation of reagents
FCR (10 mL) was taken in a 100-milliliter volumetric flask with 90
mL of water. Three grams of sodium carbonate was taken in a 50-
milliliter volumetric flask with 50 mL of water.
2.6.2. Procedure
The prepared crude extracts from the stems of neem (4 mg) were
taken in separate test tubes and 4 mL of methanol was added. A total
of 200 μL of solutions from each crude extract was taken in a test
tube and 1.5 mL of FCR was added and then kept in dark place for 5
min. After dark, 1.5 mL of sodium carbonate was added to the same
solution and mixed by hand. All the tubes were kept at dark place for
2 h. Finally, the absorbance was recorded by using UV-visible at the
wave length of 760 nm