The data reported in this study is based on observation
made after four subculture cycles.
The observations show that
continuous subculture on medium with TDZ concentration
of above 0.025 mg/L resulted in hyperhydricity.
It is believed that if subculture is carried out on medium devoid of TDZ
upon initial culture on medium with TDZ, the problemof hyperhydricity can be curbed. Furthermore, increased
callus formation and fresh weight at high concentrations of
TDZ was prominent. The outcome of the present research
complies with that of Mondal et al. [13] who found that
while the responsive explants were progressively grown on
the medium possessing TDZ, an overall callus overgrowth
and later necrosis were observed. Furthermore, this finding
is in accordance with that Bohmer et al. [21] and Murthy et
al. [22] showing the adverse influence of constant exposure to
TDZ on the multiplication and growth of pea and chickpea.
The recent achievements regarding the adverse effect of TDZ
can be best described by virtue of the capacity of TDZ in
stimulating endogenous cytokinin biosynthesis or altering
cytokinin metabolism [23, 24], and it is necessary to remove
TDZ in subsequent subcultures in order to increase the
multiplication rates or the sturdiness of the shoot. In contrast,
the findings of the current study showed that the continuous
presence of BAP is imperative for shoot multiplication media.
Constant presence of BAP at optimal concentrations was
necessary at different subcultures and there seemed to be a
cumulative gain over subcultures in both the number as well
as the sturdiness of the shoots. These results clearly showed
that TDZ was inferior to BAP in clone Iran 100.