The ability to continuously monitor dissolved oxygen
levels in our shake flask cultures demonstrated the critical
requirement for low culture volume (50 mL) and high shaking
frequency (350 rpm) to ensure oxygen availability
which increased rates of culture growth and blastospore
yields (Table 1; Fig. 1). Oxygen availability is dependent
on the oxygen transfer into the liquid, which is increased
through the use of baffled flasks and high agitation speeds.
More interestingly, we have shown the unprecedented evidence
for high glucose concentrations sustaining higher dissolved
oxygen levels in the culture broth of a filamentous