No Enterobacteriaceae were detected during the storage period
in both crab tissues, whereas H2S-producing bacteria were only
detected after day 7 in LF tissues, reaching 3.5 log CFU/g at the end
of the experiment. TVC and Pseudomonas spp. were below
4 log CFU/g until day 4 in all crab treatments and tissues. There-
after, TVC and Pseudomonas spp. growth was considerably faster in
both DR tissues and in LF muscle compared to the remaining
treatments (Fig. 7). Such differences suggest that post-mortem
changes in dead animal tissues and freezing/thawing-induced
variations favoured microbial growth, likely due to an increase of
tissue drip loss and protein denaturation, leaching out dissolved
nutrients that can be used by bacteria (Jiang & Lee, 2004). In
contrast, bacterial growth in LR tissues and LF brown meat was
reduced throughout the experiment, likely due to the lower
availability of nutrients for bacterial growth (Fig. 7). At the end of
the experiment, only DR tissues exceeded the acceptability limit of
6 log CFU/g (established for cooked, refrigerated and frozen crab
meat by ICMSF, 1986).
No Enterobacteriaceae were detected during the storage period
in both crab tissues, whereas H2S-producing bacteria were only
detected after day 7 in LF tissues, reaching 3.5 log CFU/g at the end
of the experiment. TVC and Pseudomonas spp. were below
4 log CFU/g until day 4 in all crab treatments and tissues. There-
after, TVC and Pseudomonas spp. growth was considerably faster in
both DR tissues and in LF muscle compared to the remaining
treatments (Fig. 7). Such differences suggest that post-mortem
changes in dead animal tissues and freezing/thawing-induced
variations favoured microbial growth, likely due to an increase of
tissue drip loss and protein denaturation, leaching out dissolved
nutrients that can be used by bacteria (Jiang & Lee, 2004). In
contrast, bacterial growth in LR tissues and LF brown meat was
reduced throughout the experiment, likely due to the lower
availability of nutrients for bacterial growth (Fig. 7). At the end of
the experiment, only DR tissues exceeded the acceptability limit of
6 log CFU/g (established for cooked, refrigerated and frozen crab
meat by ICMSF, 1986).
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