Figure 1. Deduced amino acid sequence of NtCDPK1 and alignment with related sequences. The deduced amino acid sequence of NtCDPK1
was aligned with the sequence from DcCPK1 of carrot [31], OSCPK2 of rice [6], and SK5 of soybean [13]. The number on the right indicates
the amino acid residues. Gaps, indicated by dashes (−), were introduced to maximize alignment. Identical amino acid residues are indicated by
dots (.). The boundaries of the variable domain, kinase domain, junction domain, and calmodulin-like domain are indicated. The amino acid
residues corresponding to the degenerate oligonucleotides (IHRDL, sense primer: DVWSF, antisense primer) for PCR amplification are marked
with arrows and shades. Asterisks (∗) indicate residues that are conserved in all serine/threonine-type kinases. The N-terminal Pro-Gln-rich
sequence is marked with a box