Frozen shrimp nuggets were defrosted at ambient temperature and then homogenized using a Waring blender for about 1 min 50 g of each grinded sample was put into a 500 ml beaker and 300 ml of n-hexane (ratio product: solvent 1:6) added. Extractions were carried out by stirring at 50 องศาเซลเซียส for 3 h. After extraction, the suspensions were filtered (Whatman paper No. 1) and the solvent evaporated by a Rotavapor R114 (Bu chi, Switzerland).