Bacteria isolated from the rhizosphere, root samples were shaken
vigorously to remove loosely adhering soil and 4.5 ml of sterile physiological
water was added to 0.5 g of rhizospheric soil and the mixture
was shaken at 120 rpm for 2 min. Serial ten-fold dilutions were prepared
from the extract and 0.1 ml of each dilution was seeded onto
King B medium, supplemented with 100 μg ml-1 of cycloheximide to
suppress fungi. To isolate and quantify Pseudomonads fluorescens UV
light was used [7].