Cultured brain cells treated with Pb (4.8 and 7 mM for
50 and 100 ppm groups, respectively) and Hg (2.4 and 23
μM for 50 and 80 ppm groups, respectively) for overnight,
were then fixed, incubated with anti-tubulin and anti-tau
antibody and subsequently visualized with ABC reagent (Vector
Laboratory, Burlingame, USA). All imaging data were processed
with light-microscopy (×200) (PC-2, Olympus, Tokyo, Japan).
Brain cells cultured treated with Lead (4.8 and 7 mA for
50 and 100 PPM Group respectively) and Mercury (2.4 and 23
microns, 50 and 80 PPM Group, respectively)
has been fixed for Overnight, incubated with Protection. tubulin and prevents -tau.
containing antibodies and a Subsequent ABC
(Vector Laboratories, Berlin, United States) Imaging Data that is Processed by an Optical Microscope (× 200) (PC-2, Olympus, London. Tokyo, Japan).
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Cultured brain cells to PB (4 and 7 mm. For
50 and 100 ppm groups, respectively), and mercury (2.4 and 23
mu M 50 and 80 ppm groups, respectively) for overnight
edited incubated with antibodies against resistance. Tubulins gray
and later phenomenon with ABC Reagent (Vector
Laboratory, Tel Aviv, USA) image data is processed
by an optical microscope (× 200) (pc-2, Olympus, Tokyo, Japan).
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