In this study, we focused on hydrophilic antioxidants potentially
present in the polar-soluble crude extracts from defatted sesame
seeds. Therefore, we attempted to separate the polar-soluble
crude extracts into different saccharide fractions. Extracts were
analyzed by HPLC gel filtration column chromatography (YMCPack
Diol 60) and the eluted saccharide fractions were identified
using a refractive index detector (RID). Chromatograms of white
and gold sesame seed extracts are illustrated in Fig. 3A and B. Both
extracts were separated into two main saccharide fractions. The
eluted peaks were identified by comparison with standard oligosaccharides
separated under the same HPLC conditions (Fig. 3C).
The first fraction, designated as F1 was obtained with 17.1% and
17.5% yield from white and gold sesame seed polar-soluble crude
extracts, respectively. This fraction corresponded to relatively high
molecular weight saccharides, such as polysaccharides or othercomplex molecules. The second fraction (F2) was obtained with
73.8% and 75.2% yield from white and gold sesame seed polar-soluble
crude extracts, respectively. This fraction consisted of different
oligosaccharides ranging from di- to hexasaccharides
consistent with the oligosaccharide composition of sesame seeds
reported previously as sucrose, raffinose, stachyose, planteose, sesamose,
as well as penta- and hexasaccharides (Wankhede &
Tharanathan, 1976).
In this study, we focused on hydrophilic antioxidants potentiallypresent in the polar-soluble crude extracts from defatted sesameseeds. Therefore, we attempted to separate the polar-solublecrude extracts into different saccharide fractions. Extracts wereanalyzed by HPLC gel filtration column chromatography (YMCPackDiol 60) and the eluted saccharide fractions were identifiedusing a refractive index detector (RID). Chromatograms of whiteand gold sesame seed extracts are illustrated in Fig. 3A and B. Bothextracts were separated into two main saccharide fractions. Theeluted peaks were identified by comparison with standard oligosaccharidesseparated under the same HPLC conditions (Fig. 3C).The first fraction, designated as F1 was obtained with 17.1% and17.5% yield from white and gold sesame seed polar-soluble crudeextracts, respectively. This fraction corresponded to relatively highmolecular weight saccharides, such as polysaccharides or othercomplex molecules. The second fraction (F2) was obtained with73.8% and 75.2% yield from white and gold sesame seed polar-solublecrude extracts, respectively. This fraction consisted of differentoligosaccharides ranging from di- to hexasaccharidesconsistent with the oligosaccharide composition of sesame seedsreported previously as sucrose, raffinose, stachyose, planteose, sesamose,as well as penta- and hexasaccharides (Wankhede &Tharanathan, 1976).
การแปล กรุณารอสักครู่..
