one of the main enzymes responsible for polymer begradation was identified,and the biodegradation mechanism was hypothesized by bioinformatics studies.
From this study, it is evident that the bacteria utilized the plastic polymer as a sole source of carbon and showed 20-50% weight reduction over a period of 120 days.
The two main bacteria responsible for the degradation were microbiologically characterized to be Pseudomonas spp.
These bacteria could grow optimally at 37 ํC in pH 9.0 and showed 35-40% of plastic weight reduction over 120 day.
plastic weight reduction over a period of 90 days.
These isolates were showed better degradation ability than Known strains from MTCC.
The current study further revealed that the microbial consortia formulated by combinnig Psuedomonas spp.
showed 40% plastic weight reduction over a period of 90 days.
Further,extracellular lipase, one of the main enzymes responsible for polymer degradation, was identified.
The computational docking studies suggested that polyethylene glycol and polystyrene present in the plastic might have good interaction towards the microbial lipase with stable binding and interacting forces which probably could be one of the reasons for the
degradative mechanisms.