Preparation of alcohol/salt Aqueous Two Phase System [ATPS]:
Five mL distilled water, 2.0 mL alcohol, a given amount of salt and 0.1 mL aloe AQs solution were added to. The mixture was stirred well to make the salt dissolve completely. The separation phase could be achieved during a few seconds, two clear phases formed. The uper phase was mainly composed of alcohol and AQs with a small volume, and the lower phase was the salt-rich solution containing impurities with large volume. The volume of each phase was noted down. The alcohol-rich phase was withdrawn to another tube using a syringe, then diluted using 0.5 % magnesium acetate–methanol solution, the AQs solution turns to red color under this weakly basic condition. The total AQs concentration was determined by colorimetric method using a UV–Vis spectrophotometer.
Preparation of alcohol/salt Aqueous Two Phase System [ATPS]:Five mL distilled water, 2.0 mL alcohol, a given amount of salt and 0.1 mL aloe AQs solution were added to. The mixture was stirred well to make the salt dissolve completely. The separation phase could be achieved during a few seconds, two clear phases formed. The uper phase was mainly composed of alcohol and AQs with a small volume, and the lower phase was the salt-rich solution containing impurities with large volume. The volume of each phase was noted down. The alcohol-rich phase was withdrawn to another tube using a syringe, then diluted using 0.5 % magnesium acetate–methanol solution, the AQs solution turns to red color under this weakly basic condition. The total AQs concentration was determined by colorimetric method using a UV–Vis spectrophotometer.
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