Biological and chemical pretreatment methods using LFD, AS,
and NaOH were compared. LFD pretreatment could achieve the
same effect as the chemical pretreatment (AS, NaOH) at the performance
of AD. Compared with the untreated corn stover, the CBP
and the VS removal rate of three pretreatment methods were
increased by 25.40–30.12% and 14.48–16.84%, respectively, in the
co-digestion of cattle manure and corn stover. T80 was 20–37.14%
shorter than that of the control test (35 ± 1 days). LFD pretreatment
not only achieved the same effect as chemical pretreatment,
but also reduced T80 and improved buffer capacity of AD system.