Interaction of cationic antimicrobial (ɛ-polylysine) with food-grade biopolymers: Dextran, chitosan, carrageenan, alginate, and pectin
The cationic biopolymer ε-polylysine (ε-PL) is a food-grade antimicrobial that is highly effective against food
pathogens and spoilage organisms. In compositionally complex environments, the antimicrobial activity of cationic
ε-PL is likely to be impacted by its interactions with other charged species. The purpose of this study was
to characterize the interactions of cationic ε-PL with various food grade biopolymers with different charge characteristics:
anionic (carrageenan, alginate, pectin), neutral (dextran), and cationic (chitosan). Isothermal titration
calorimetry (ITC), micro-electrophoresis (ME) and turbidity measurements were used to characterize the interactions
and the nature of any aggregates formed. Our measurements suggested that there was little interaction or
complex formation between cationic ε-PL and cationic or neutral biopolymers, but that strong electrostatic interactions
and complex formation occurred between cationic ε-PL and anionic biopolymers. The solubility of the aggregates
formed depended on biopolymer type and the mass ratio of biopolymers to ε-PL. The results of this
study have important implications for the application of ε-PL in compositionally complex systems.
Interaction of cationic antimicrobial (ɛ-polylysine) with food-grade biopolymers: Dextran, chitosan, carrageenan, alginate, and pectinThe cationic biopolymer ε-polylysine (ε-PL) is a food-grade antimicrobial that is highly effective against foodpathogens and spoilage organisms. In compositionally complex environments, the antimicrobial activity of cationicε-PL is likely to be impacted by its interactions with other charged species. The purpose of this study wasto characterize the interactions of cationic ε-PL with various food grade biopolymers with different charge characteristics:anionic (carrageenan, alginate, pectin), neutral (dextran), and cationic (chitosan). Isothermal titrationcalorimetry (ITC), micro-electrophoresis (ME) and turbidity measurements were used to characterize the interactionsand the nature of any aggregates formed. Our measurements suggested that there was little interaction orcomplex formation between cationic ε-PL and cationic or neutral biopolymers, but that strong electrostatic interactionsand complex formation occurred between cationic ε-PL and anionic biopolymers. The solubility of the aggregatesformed depended on biopolymer type and the mass ratio of biopolymers to ε-PL. The results of thisstudy have important implications for the application of ε-PL in compositionally complex systems.
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