Progressive adaptation of Zymomonas mobilis in soybean molasses was done through successive transferences from the seed culture (10% inoculation rate). Initially, it was used soybean molasses at 50 g L-1 of soluble solids (first transference), and thereafter (second and third transferences) soybean molasses at 100 g L-1 of soluble solids. The stock culture was stored for no longer than 3 weeks at 4 °C. These cultures were grown at the same conditions described previously.