A volume of 5.0 mL of water
was used for cleanup. The next step included drying under a mild nitrogen
flush. Chloroform (2 0.5 mL) was used for fatty acid elution. For the
determination of medium-chain fatty acids (C6-C12) 2 μL of this chloroform
extract was injected to the GC column. For the determination of
long-chain fatty acids (C14-C18) the chloroform extract was evaporated