. For other analytes just very
minor decrease in retention was observed with increasing
temperature in the relatively wide range from 5 to 60 °C.
This was observed, for instance for the enantiomers of
ibuprofen enantiomers on Lux Cellulose-1 in MeOH as
mobile phase and for ketoprofen enantiomers on Lux Cellulose-
2 in the same mobile phase (data not shown). Only
few examples have been reported in the literature where
the retention of chiral analytes stayed (mostly) unchanged
or even increased while the separation selectivity and peak
resolution improved with increasing temperature [42, 43].
Quite interesting example was observed for separation
of surprofen enantiomers on the ADMPC-based column
in ACN as the mobile phase. For this analyte, the retention
and separation selectivity (the latter only slightly)
decreased with increasing temperature while peak efficiency
increased significantly. As a result of this interplay