After modification with the DNA aptamer monolayer, the modified
electrode was used to recognize various concentrations of
different signal-stranded DNA diluted with 1×
SSC solution by incubation
at room temperature for 1 h. After incubation, the modified
electrode was then rinsed with PBS and DDW to remove the free
DNA oligonucleotides. The electrode was then immersed in a 10 M
solution of indicator for 20 min to perform electrochemical detection.