This method involved
removal of protein with pepsin (40 C, 1 h, pH 1.5), incubation with
a-amylase (37 C, 16 h) to hydrolyse digestible starch, treatment of
the residues with 2 M KOH to solubilise RS and finally incubation
with amyloglucosidase (60 C, 45 min, pH 4.75) to hydrolyse RS.