As aglycogen antibody from a different host species to that used for
pGS staining was unavailable, an alternative method was
devised in which alkoxyamine-biotin coupling with FITCstreptavidin
was used in place of Schiff’s reagent for
fluorescent staining of periodate-oxidized glycogen and colocalization
with pGS in confocal microscopy (see
Supplementary Methods and Supplementary Fig. S5D)