Incubation temperature of a BMP test is usually 35oC (i.e., the optimum temperature for mesophyllic organisms). Other temperatures can be used depending on operating temperature of the proposed digester. Total analysis size is usually 200 ml, with a variable substrate VS to seed VS mass ratio (1:1 to 2:1) based on individual substrate characteristics. Substrate VS to seed VS ratio is the same when testing both solid and liquid substrates.