3,5-Dinitrosalicylic acid (DNS) reagent is widely used in the estimation of reducing sugars.
The reagent shows a differential behaviour towards mono- and di-saccharides. This
phenomenon has been misinterpreted in the literature. Contrary to the facts, it has been
reported that the DNS test is less sensitive for the estimation of cellobiose than it is for the
estimation of glucose. This communication clarifies the concept. In addition, the study also
compares the reaction of different mono- and di-saccharides and discusses the difference
in their reactivity