At the end of the experiment,
the disease incidence on each infected grape was calculated as follows:
incidence (%) = (number of decayed wounds/number of total
wounds) × 100. A positive control
was included as well as two different negative controls: wounded
grapes with 40 L of sterile distilled water and wounded grapes
with 20 L of yeast suspension and 20 L of sterile water.
Each experiment used 18 berries per replicate and three replicates per
treatment in a randomized complete block design. A reduction in
disease incidence of 60% or more was considered the selection criterion
for antagonistic yeasts. The experiment was repeated twice