The botanicals used in this experiment were, Osmium sanctum L. (Lamiaceae), Azadirachta indicia A. Joss. (Meliaceae),Caltrops gigantean R. Br. (Asclepidaceae), Nicotine abacus Linn. (Solanaceae ) and Allium sativa Linn. (Amaryllidaceous). Leaves of Osmium sanctum (Tulsan) and Azadiracta indicia (Need) and Caltrops sp. were collected from the home gardens of Batticaloa. The dried leaves of Tobacco (Nicotine Tabaco) and Garlic cloves were bought from local market.
The leaves collected were washed with sterile distilled water until the dirt was completely removed and allowed to shade dry for one week. Then the dried materials were finely ground using motor and pestle until the powdered form was obtained. The powders were stored separately in dark bottles for extraction