Cholangiocarcinoma is a fatal disease which is highly resistant to anticancer drugs.
It is noted that the IC50 values of 5-FU observed in the present study and the previous study [19] were much higher than those reported in other cancerous cell lines, e.g., colon carcinoma (HCC-48, COLO20) [20] and cervical squamous carcinoma (SiHa, HeLa) cell lines [21].