The aim of this work was to evaluate the antimicrobial activity of whey protein isolate (WPI) films
supplemented with Lactobacillus sakei NRRL B-1917 cell-free supernatant on beef inoculated with
Escherichia coli ATCC 25922 or Listeria monocytogenes Scott A; additionally, sensory evaluation was
performed on wrapped beef cubes. Supernatant concentrates were obtained from Lb. sakei cultures in
MRS broth after centrifugation, filtering, and freeze-drying. Films were prepared with WPI (3% w/w),
alginate (0.625% w/w), rehydrated supernatant (18 mg/ml), and glycerol. Films were used to wrap beef
cubes inoculated with z103 CFU/g E. coli or L. monocytogenes. Sensory evaluation was carried out on
grilled beef wrapped or not with the studied antimicrobial films. During refrigerated storage, antimicrobial
films reduced 1.4 log10 CFU/g of L. monocytogenes after 120 h, while E. coli decreased 2.3 log10 CFU/
g after 36 h. Grilled beef wrapped with antimicrobial film was well accepted by panelists, besides scores
evidenced no significant differences (p > 0.05) between wrapped and unwrapped beef.
© 2016 Elsevier Ltd. All rights reserved
The aim of this work was to evaluate the antimicrobial activity of whey protein isolate (WPI) filmssupplemented with Lactobacillus sakei NRRL B-1917 cell-free supernatant on beef inoculated withEscherichia coli ATCC 25922 or Listeria monocytogenes Scott A; additionally, sensory evaluation wasperformed on wrapped beef cubes. Supernatant concentrates were obtained from Lb. sakei cultures inMRS broth after centrifugation, filtering, and freeze-drying. Films were prepared with WPI (3% w/w),alginate (0.625% w/w), rehydrated supernatant (18 mg/ml), and glycerol. Films were used to wrap beefcubes inoculated with z103 CFU/g E. coli or L. monocytogenes. Sensory evaluation was carried out ongrilled beef wrapped or not with the studied antimicrobial films. During refrigerated storage, antimicrobialfilms reduced 1.4 log10 CFU/g of L. monocytogenes after 120 h, while E. coli decreased 2.3 log10 CFU/g after 36 h. Grilled beef wrapped with antimicrobial film was well accepted by panelists, besides scoresevidenced no significant differences (p > 0.05) between wrapped and unwrapped beef.© 2016 Elsevier Ltd. All rights reserved
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