For nuclease p1 production, the bacteria was grown at 30 8C in a medium
(pH 7.0) consisting of (w/v): sucrose 3%, potassium dihydrogen phosphate
0.10%, ferrous sulfate 0.01%, sodium nitrate 0.30%, magnesium sulfate 0.05%,
potassium chloride 0.05%, and potato extract 20%.