Based on previous results, a few C

Based on previous results, a few C sources (glucose, sucrose, maltose,
apple pectin, citrus pectin and malic acid), N sources (NaNO3,
(NH4)2SO4, arginine, glutamic acid, peptone, and yeast extract) and
pH values (3.0, 5.0, 7.0 and 8.0) were selected as cultural conditions
for gene expression analysis of the other 14 genes involved in patulin
biosynthesis in P. expansum T01. Overall, the expression profiles of
most of these genes were consistent with patulin production. In particular,
expressions of PatD, PatE, PatF, PatG, PatH, PatI, PatJ, PatK, PatM,
PatN and PatO were up-regulated in P. expansum T01 cultured in
media with glucose, maltose, glutamic acid and yeast extract as C or N
sources, which permitted the production of patulin; by contrast, these
genes were dramatically down-regulated when P. expansum T01 was
cultured with unfavorable C or N sources, such as malic acid and ammonium
sulfate (Fig. 5A, B). Similarly, except PatC and PatH, all the other 12
genes demonstrated the highest relative expressions at pH 5.0, the optimal
pH value for patulin production in P. expansum T01

ผลลัพธ์ (ไทย) 2:

Based on previous results, a few C sources (glucose, sucrose, maltose,
Apple pectin, Citrus pectin and malic acid), N sources (NaNO3,
(NH4) 2SO4, arginine, glutamic acid, peptone, and yeast Extract) and
pH values. (3.0, 5.0, 7.0 and 8.0) were selected as Cultural conditions
for Gene Expression Analysis of 14 genes involved in the Other patulin
biosynthesis in P. expansum T01. Overall, the Expression profiles of
genes Most of these were consistent with patulin Production. In particular,
Expressions of PatD, pâte, PatF, PatG, PATH, Pati, PatJ, PatK, PatM,
were up-regulated in PatN and PATO P. expansum T01 Cultured in
Media with glucose, maltose, glutamic acid and yeast as Extract C. or N
sources, which permitted the Production of patulin; by contrast, these
genes were dramatically down-regulated when P. expansum was T01
Cultured with Unfavorable C or N sources, such as malic acid and ammonium
sulfate (Fig. 5A, B). Similarly, except Patc and PATH, all the Other 12
genes demonstrated the highest Relative Expressions at pH 5.0, the Optimal
pH VALUE Production for patulin in P. expansum T01.