MATERIALS AND METHODS
Fungal strains
A total of 360 clinical yeast strains were obtained from nail infections of patients at Tehran, Isfahan, Alborz, Kashan, and Mazandaran provinces of Iran, from April 2009 to June 2011. All strains were cultured on sabouraud glucose agar (Difco, Detroit, MI, USA) and incubated at 28-30°C for 48 h. A loopful of single and fresh colonies of each strain was transferred to glycerol 20% and stored at –20°C prior to use.