In the present study, to reduce production costs, we enhanced Lipase GH1 production in a recombinant P. pastoris strain cultured in a 7.5-L fermenter using the less expensive BSMG medium. The Lipase GH1 produced was stable. We applied Lipase GH1 directlyto catalyze the reaction of monounsaturated fatty acid (oleic acid)and glycerol to synthesize MAGs and DAGs. For this purpose, LipaseGH1 was used in the form of fermentation broth supernatant. The application of Lipase GH1 for synthesis of MAGs and DAGs expandsthe potential usefulness of this enzyme.