A total of 15 different soil samples were aseptically collected from different regions of Lucknow, India. The location of collected soil was rhizosphere of maize, wheat and rice, fish market and pond. For screening of chitinase producing bacteria, the agar medium amended with colloidal chitin was used. The medium consists of (g L−1): Na2HPO4, 6; KH2PO4, 3; NH4Cl, 1; NaCl, 0.5; yeast extract, 0.05; agar, 15 and colloidal chitin 1% (w/v). The colonies showing clearance zones on a creamish background were considered as chitinase-producing bacteria.