Analysis of intact CS and HA was performed at 30 kV using reversed polarity in 50 mM phosphate buffer (pH 3.0) produced by dissolving sodium dihydrogen phosphate in water to the 50-mM concentration and adjusting buffer replenishment mode of the CE instrument was used during electrophoresis. Samples were introduced hydrodynamically (500 mbar * s ) using the pressure injection mode. Detection was performed at 200 and 260 nm.