A single, blinded examiner performed the following histometric analyses using a microscope at 200 or 400
magnification, after the preparations were assigned random numbers. The tissue sections stained with haematoxylin and
eosin were used to evaluate histological changes. The linear distance between the cemento-enamel junction and the
alveolar bone crest was measured using a microgrid at a magnification of 200. The numbers of polymorphonuclear
leukocytes per unit area (0.1 mm 0.05 mm) of the connective tissue subjacent to the junctional epithelium were determined under a magnification of 400